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Since its introduction in 1979, protein blotting17 has become a routine tool used to detect low amounts of proteins in complex samples or to monitor protein expression and purification. The simplest protein blotting procedure, known as dot blot or slot blot, uses vacuum filtration to transfer protein onto a microporous membrane.
Dot blot technique, also called slot blot technique, is a method of identifying DNA, RNA and Protein in the sample. It involves blotting the biomolecules directly onto the filter membrane and hybridizing them with a radioactive probe.
