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Meiotic segregants carrying the gas2 or gas4 null mutation together with the genetic markers required for diploid selection were backcrossed with ER300 or ER301 to generate gas2Δ/gas2Δ (ER306), gas4Δ/gas4Δ (ER307), and gas2Δ/gas2Δ gas4Δ/gas4Δ (ER309) null diploids.

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The ectopic expression of GAS2 and GAS4 genes in a gas1 null mutant revealed that these proteins are redundant versions of Gas1p specialized to function in a compartment at a pH value close to neutral.

It encodes a glycosylphosphatidylinositol-anchored protein functioning as aβ (1,3)-glucan elongase and required for proper cell wall assembly during vegetative growth. In this study, we characterize the roles of the GAS2 and GAS4 genes. These genes are expressed exclusively during sporulation.

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